HOME CONTACT CAREERS ABOUT
 
Improvision License Server. The Server Solution for Multi-user Access

Openlab Application - Deconvolution

Openlab supports three deconvolution modules: Nearest Neighbor Deconvolution and Volume Deconvolution, which are included in your Openlab license, and 3D Restoration, which is not part of the standard Openlab package but which can be purchased as an addition to your system. Each module removes of out-of-focus haze, providing confocal quality images from a standard research microscope. For applications that require improved resolution in Z as well as in X and Y, the 3D Restoration module provides superior restoration results and uses an algorithm developed by Improvision from published Maximum Entropy techniques.

Deconvolved Openlab image z series can be imported directly into Volocity for volume rendering and further analysis.

Raw image data
Deconvolved image
 Raw and Deconvolved image merge
Raw image data
Deconvolved image

Openlab Application – 3D imaging

The Openlab 3D Bundle contains the 3D Rendering module and the 3D Slicing module. The 3D Rendering module allows you to visualize deconvolved or raw data sets in three dimensions and 24 bit real color. This module provides controls for interactive viewing of rendered images, 3D movie production and visualization of colocalized wavelengths in three dimensions. The 3D Slicing module allows you to visualize deconvolved data sets by slicing the image stack at angles orthogonal to the XY plane.

Deconvolution case study

Dr Helen Mardon's group in the department of Obstetrics and Gynecology, University of Oxford, is focused on the dissection of the cellular mechanisms of human endometrial function in health and disease. The group uses an Openlab cell imaging system for high resolution imaging of triple labeled samples.

The images shown here are of trophoblasts penetrating a monolayer of endometrial stromal cells. A Z series of images was acquired at each emission wavelength using a high resolution monochrome camera, then deconvolved to remove the out-of-focus haze using the Openlab Volume Deconvolution module. The appropriate color LUT was applied to each Z series and the images at each Z plane were then merged in Openlab to give the final three color images.