Volocity Acquisition

Volocity Acquisition is designed for high speed image capture on Mac or Windows OS. Set up your experimental protocol using the clear and easy to use Acquisition dialogue – from simple 2D capture to multi-channel 4D experiments.

You can save your protocols for future use so that experiments which you repeat regularly will be identical every time.

Use the Light Path Manager to group together multiple hardware settings, such as a filter choice with a camera exposure and camera gain, so that you can quickly and accurately move between hardware configurations.

And for quicker navigation, we’ve given you the option to hide the interface controls that you don’t need, so that the dialogue is easier to view and use.

Of course, you want to view your sample before you start to capture images. With the Volocity Video Preview window, you can do just that. You have the option to apply Auto Contrast to both the Video Preview and to the images as they are acquired. Auto Contrast allows you to set a shorter camera exposure without reducing the apparent brightness of the image, ideal for fast focusing or very rapid image acquisition. The Auto Contrast feature is a manipulation of the visual appearance of the image and does not affect the integrity of the raw data.

If you have Volocity Restoration, you can choose to view either the standard wide field image or the deconvolved image in the Video Preview by checking the ‘Fast Restoration’ button in the dialogue. For a Volocity Grid Confocal system, you will also have the option to view the grid confocal image live in the Video Preview.

By viewing the image in all the modes before you start to capture, you can decide on the best option for the sample you are currently using. Your images will also be saved in the mode in which you choose to view them.

Your images are acquired directly into an Image Sequence in a Volocity Library and you can export them as QuickTime, AVI or WMV movies. But why stop there? With Volocity Visualization you can explore your Z stacks as fully interactive 3D renderings or identify, analyze and track objects in your 2D, 3D or 4D data using Volocity Quantitation.

FRAP and Ratio Acquisition

Volocity Acquisition can be used to acquire and analysis FRAP and ratiometric imaging experiments, by adding the optional FRAP Plugin and Ratio Plugin to your system.  Volocity Quantitation must be included in the Volocity configuration in order to perform analysis.

The FRAP Plugin supports the PhotoKinesis™ Accessory, which is an option to the UltraVIEW® VoX spinning disk confocal imaging system.  This configuration provides all the tools required to acquire andanalyze FRAP images, providing both raw and graphical data. In addition to FRAP experiments, the unit can be used for techniques such as FLIP, photoswitching (photoconversion) and acceptor photobleaching.

The Ratio Plugin provides the ability to acquire 2D and 3D ratiometric image pairs, with simultaneous online analysis, or post-acquisition analysis and charting of results.