High Speed 4D Confocal Imaging - for an Advanced View on Life

In April we were pleased to announce that Improvision had become part of the PerkinElmer family. PerkinElmer Inc. has supplied the global life science community with exceptional spinning disk technology for over 10 years, providing systems that are renowned for reliability and imaging quality. Improvision is recognized worldwide as the market leading developer of 3D software solutions for biologists. Now we have combined forces to create a new, improved spinning disk system for 4D live cell imaging - the UltraVIEW^® VoX

The Next Generation in 3D Live Cell Imaging

• New spinning disk technology– very fast and very optically efficient

• Fully integrated with Volocity for 4D multi-channel image acquisition and analysis

• Modular solid-state laser unit – from one to six lines in a wide range of wavelengths

• Complete synchronization for maximum speed and superb image quality

• Versatile, easy to use system, designed to meet the challenges of live cell imaging research

High Speed Acquisition and Exceptional Image Quality
The UltraVIEW VoX includes the CSU-X1 spinning disk head, the latest technology from Yokogawa^®.

• The CSU-X1 has been engineered to provide twice the optical efficiency of the CSU 10 or 22, so that the required laser power is even lower than before, thereby ensuring even better sample protection. Even low intensity emission can be detected and captured using this highly efficient confocal system.

• Includes the PerkinElmer ProSync^™ synchronization technology, to achieve the highest acquisition rates, which synchronizes the scanning disk speed, camera acquisition, wavelength selection and Z position. Choose from a range of CCD and EMCCD cameras, selected for speed and sensitivity.

• Samples are exposed to excitation light only during image capture, to further reduce photobleaching and phototoxicity. This means that cells are less affected by the imaging process and therefore their behavior is closer to the natural state.

Modular Laser System
The UltraVIEW VoX is equipped with a Modular Laser System which uses solid-state laser technology.

• The lasers are housed in a configurable unit that does not need dedicated cooling, reducing the physical space required as well as energy costs for the system.

• Up to six lasers can be included in the unit and these can be chosen from a wide range of wavelengths for maximum flexibility. You could start with just one laser and build up to a full set of six as your science progresses.

• The lasers need minimal alignment for ease of use and have AOTF or direct on/off control for faster switching, which is critical for high speed, multi-channel time lapse experiments.

Powered by Volocity – Fully Integrated Acquisition and Analysis
The UltraVIEW VoX includes Volocity for a complete 4D image acquisition and analysis experience. Working with your samples in 3D can reveal new information and lead to a better understanding of cellular processes, as well as providing more accurate results, especially when tracking objects. Now you can acquire, analyze, create movies and images for publication, all within one software environment.

• Volocity Acquisition provides the interface to all the acquisition hardware in the system, including the objective turret, light paths, focus drive and XY stage on any motorised microscope. Setting up the acquisition protocol is quick and easy, and protocols can be saved for repeated use. For time lapse experiments, once the first time point has been acquired you can immediately begin to review the image data, so that you can be sure your protocol is working right away.

• Volocity Visualization provides a choice of ways to explore your 3D and 4D image data – as XYZ slices and as high resolution rendered volumes. Fully interactive, even with 3D time lapse data. See your samples as you never have before!

• Volocity Quantitation provides a comprehensive set of tools to measure, analyze and track objects in 3D. Measurement analysis features are included to summarise and extract data, and charts and graphs can be automatically created for export and publication, all within Volocity.

FRAP PhotoKinesis Unit - Acquire and Analyze in One System
Add the optional FRAP PhotoKinesis™ Unit, which is fully integrated with the system and controlled by Volocity. This provides all the tools needed to acquire and analyze FRAP images, providing both raw and graphical data. The PhotoKinesis™ Unit supports the unique “Track-It” technology, which allows you to respond to changes and events in live samples, so that you can achieve the maximum information from each and every cell. In addition to FRAP experiments, the unit can be used for techniques such as FLIP, photoswitching (photoconversion) and acceptor photobleaching.

For more information about the UltraVIEW VoX please use the contact form.